Scientist Solutions: International Life Science Community By Scientists For Scientists
Home » Protocols » An Agar Mount for Observation of Caenorhabditis elegans Embryos
Thanks to our sponsors who make this site possible
Protocol for

An Agar Mount for Observation of Caenorhabditis elegans Embryos

Would you like to save this topic, event, protocol or job so you can find it again easily?

Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space.

Available to members only. Please log in or register for your free account now.

Abstract or Description:

The Caenorhabditis elegans embryo is particularly amenable to microscopy and embryological studies because of its short developmental time, transparent shell, and nonpigmented cells. The agar mount described in this protocol is an easy way to prepare live C. elegans embryos for microscopic visualization. The mount slightly embeds the embryo in agar to hold it in place. The mount also slightly compresses the embryo to provide consistent orientation such that every embryo will be positioned with either its right side or its left side facing the objective. Other techniques can result in random orientations that complicate analysis and make identification of individual blastomeres more challenging.

Attachments: No Attachments
Posted By: dacrotty on 12/8/2010 4:05:10 PM
Add Comment