Protocol for: Protocol for labeling apoptotic cells in vivo. Microscopy
Link to Full Protocol: http://www.vergentbio.com/resources/protocol-in-vivo-apoptosis-microscopy
Abstract or Description:
Label apoptotic cells in vivo using CAS-MAP™ Apoptosis probes. The probe is injected into the animal IV at the time when you expect apoptosis to be occuring in the animal. The apoptosis probes label caspase activity inside the cell. CAS-MAP is cell permeable, non toxic and easy to use. Researchers can now detect apoptosis in vivo and analyze by microscopy. The advantage is that terminal processing procedures can induce some apoptosis. Staining methods after termination will detect artificial apoptosis that did not occur in vivo. This protocol will guide you on setting up an experiment where apoptotic cells are labeled in vivo, then tissues are excised and visualized by microscopy

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Posted By: amyclausen on 10/2/2015 5:53:18 PM