Scientist Solutions: International Life Science Community By Scientists For Scientists
    
Home » Protocols » Protocol for labeling apoptotic cells in vivo. Microscopy
Thanks to our sponsors who make this site possible
Protocol for

Protocol for labeling apoptotic cells in vivo. Microscopy

Would you like to save this topic, event, protocol or job so you can find it again easily?

Just click the "Save to My Lab Drawer" link and the item will be saved in the My Lab Drawer section of your bench space.

Available to members only. Please log in or register for your free account now.

Abstract or Description:

Label apoptotic cells in vivo using CAS-MAP™ Apoptosis probes. The probe is injected into the animal IV at the time when you expect apoptosis to be occuring in the animal. The apoptosis probes label caspase activity inside the cell. CAS-MAP is cell permeable, non toxic and easy to use. Researchers can now detect apoptosis in vivo and analyze by microscopy. The advantage is that terminal processing procedures can induce some apoptosis. Staining methods after termination will detect artificial apoptosis that did not occur in vivo. This protocol will guide you on setting up an experiment where apoptotic cells are labeled in vivo, then tissues are excised and visualized by microscopy

Citation:
Attachments: No Attachments
Posted By: amyclausen on 10/2/2015 5:53:18 PM
Comments
Add Comment
       
Click here to
Become a member & join our
community (It's easy & free)
Already a member? Please log in
User Name  
Password  
Forget Password?
Scientists
Post a new protocol

You must be logged in to post. Log in above.
Not a member yet? Click here to register
(it's free)
Thank You to Our Sponsor

The Latest from our Forums

The Latest Events

No events found.