Protocol for: Label apoptosis in vivo and analyze by flow cytometry
Link to Full Protocol: http://www.vergentbio.com/resources/protocol-in-vivo-apoptosis-flow-cytometry
Abstract or Description:
CAS-MAP imaging probes are injected IV into the animal to label active caspase-positive apoptotic cells. The probes are cell permeant and non-toxic. By labeling apoptotic cells in vivo, only apoptotic cells at the time of injection are labeled. Unbound probe is removed by the circulatory system. This eliminates any false positives that are typically labeled due to post-terminal staining methods. This protocol will guide researchers to set up the animal experiment and label apoptosis in vivo with analysis by flow cytometry.
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Posted By: amyclausen on 10/2/2015 5:59:40 PM