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Region and cell-specific expression of a gene of interest is a critical experimental consideration, especially when studying the human brain which by some estimates contains 100 billion cells and poss
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In 2011, Addgene began collaborating with Dr. Alex Chenchik of Cellecta, Inc. and Dr. Gus Frangou of the Fred Hutchinson Cancer Research Center to distribute Cellecta's DECIPHER pooled lentiviral sh
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  Protein kinases post-translationally phosphorylate specific residues as a means of regulating cell-signaling cascades. O-phosphoserine (Sep) being, by far, the most common phosphorylation modi
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 This is a thorough explanation of how to use the free densitometry software ImageJ for western blot quantification.  It covers proper exposure of film and relative comparisons to analyze b
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 This is a full western blot protocol using the standard tris-glycine-SDS running buffer and tris-HCl buffered gels. Full gel recipes are provided along with compostions, ingredients, and recipes
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Optical molecular imaging techniques have emerged as valuable tools for pre-clinical oncology research in mouse models of cancer.1-5 Diseased tissues and cells are typically detected during in vivo fl
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 This is part one of a troubleshooting guide to fix your Western blots.   3 hrs and 33 minutes are needed to complete the protocols.   Protocol written and proven by westernblot.o
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This document contains recommendations on Morpholino oligo stock solution concentrations, storage temperature and conditions, as well as notes on lyophilization for long-term storage.
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 Deglycosylation Using TFMS   Glycosylation is one of the most common posttranslational modifications of proteins. Glycans attach to the peptide backbone through either amide or glycosidic
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Mouse Embryonic Stem Cells cultured on a matrigel-coated 100 mm dish have been transfected with GeneCellin HTC. We have used 2x106 cells in a 100 mm dish, 15 µg of plasmid DNA and 30
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