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Targeting stem cells using surface-engineered lenti vectors

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parvoman
Scotland

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Topic Started by parvoman
on 8/8/2009 18:38 PM   
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A number of interesting recombinant viral vector protocols have been published by Cold Spring Harbor Protocols. This one describes the use of lenti viruses that have surface-engineered coats to target hematopoietic stem cells.

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I-R-Scientist
United States

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Posted By I-R-Scientist
on 8/20/2015 0:48 AM   
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An interesting protocol, for sure.  Unfortunately, it doesn't look like the plasmids expressing the polypeptide fusion envelope proteins are available.

I was thinking of trying the RD114:MLV-A fusion envelope protein available on Addgene: https://www.addgene.org/17576/

It doesn't promise quite what the activating peptides in the CSH protocol do, but apparently shows high transduction and reduced toxicity compared to VSV-G.




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